Hi Cl3arpass12,
Without knowing the config of the switch, this is hard to say.
If you just enable user roles globally, nothing will happen on the ports, because you need to enable authentication on a per-port basis as well.
So enabling user roles globally will not change any port, but if you then enable dot1x or mac auth on a specific port, I#m not really sure if the user will disconnect or not. I would assume the user will disconnect to do authentication.
Afterward, every user needs to authenticate against the radius server and will get access to the network or not.
you should have a look into the security guide of your switch for more details.